Total Human IgG ELISA Kit | F160
- SKU:
- 214-F160
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Assay format:
- 96 Well Plate
- Time to result:
- ~1 hr. 50 min
- LOD:
- 0.1 ng/mL
- LLOQ:
- ~0.2 ng/mL
- Recommended Diluent:
- I028
Description
Total Human IgG ELISA Kit | F160
Total Human IgG ELISA Kit is intended for use in quantifying very low concentrations of total human immunoglobulin G (hIgG). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The hIgG assay is a two-site immunoenzymetric assay. Samples containing hIgG are reacted in microtiter strips coated with an affinity purified capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled anti-hIgG antibody, is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody- hIgG -enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of hIgG present.
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