Total Human IgG ELISA Kit | F160

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SKU:
214-F160
Size:
1 Kit
Product Type:
ELISA Kit
Storage:
All kit reagents 2°C to 8°C
Assay format:
96 Well Plate
Time to result:
~1 hr. 50 min
LOD:
0.1 ng/mL
LLOQ:
~0.2 ng/mL
Recommended Diluent:
I028
  • Total Human Kit
  • Total Human IgG ELISA Kit
€1,200.00
Frequently bought together:

Description

Total Human IgG ELISA Kit | F160

Total Human IgG ELISA Kit is intended for use in quantifying very low concentrations of total human immunoglobulin G (hIgG). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The hIgG assay is a two-site immunoenzymetric assay. Samples containing hIgG are reacted in microtiter strips coated with an affinity purified capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled anti-hIgG antibody, is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody- hIgG -enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of hIgG present.

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