Borrelia burgdorferi RT-PCR (CE) | B37-FRT

Borrelia burgdorferi RT-PCR | B37-FRT from Sacace Biotechnologies is available for delivery

Real Time PCR Kit for qualitative detection of Borrelia burgdorferi in biological materials

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

Borrelia burgdorferi RT-PCR (CE) B37-FRT DataSheet

INTRODUCTION

Lyme disease (LD) is a vector-borne, multisystem inflammatory disease caused by the spirochete Borrelia burgdorferi sensu lato. It is transmitted to humans by infected tiks of the Ixodes genus. After entering the circulation, the organism invades the cutaneous, synovial, cardiac, and nervous system. Spirochetes have also been demonstrated histologically in bone marrow, the spleen, lymph nodes, the liver, testes, and the placenta during early hematogenous dissemination.

INTENDED USE

Kit Borrelia burgdorferi Real-TM is an in vitro nucleic acid amplification test for qualitative detection and identification of Borrelia burgdorferi in the biological materials. The strains detected by the kit Borrelia burgdorferi Real-TM are the followings: B.burgdorferi, B.afzelii, B.garinii, B.valaisiana, B.tanukii, B. bissetii, B.americana, B.spielmanii, B.sinica, B.lusitaniae, B.andersonii, B.turdi, B.japonica.

PRINCIPLE OF ASSAY

Kit Borrelia burgdorferi Real-TM is based on two major processes: isolation of DNA from specimens and Real Time amplification. Borrelia burgdorferi DNA and Internal Control (IC) DNA are extracted from the specimens, amplified using Real-Time amplification and detected by fluorescent reporter dyes linked to hydrolysis probes specific for Borrelia burgdorferi DNA and IC. IC serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition. IC is detected in a channel other than the Borrelia burgdorferi.