A.I.I. Screen RT-PCR (CE) | B45-FRT

A.I.I. Screen RT-PCR (CE) | B45-FRT from Sacace Biotechnologies is available for delivery

Real Time PCR test for the qualitative detection and differentiation of Shigella Spp. E.coli, Salmonella spp., Campylobacter spp., Adenovirus F, Rotavirus A, Norovirus 2 genotype, Astrovirus

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

A.I.I. Screen RT-PCR (CE) B45-FRT DataSheet

INTRODUCTION

Acute Intestinal Infections (A.I.I) are one of the primary causes of hospitalization in infectious disease departments. In accordance with the data provided by the contemporary literature the following bacterial and viral agents are the most often detectable and generally spread etiological agents of A.I.I.:

Bacterial agents:

• Shigella species microorganisms and enteroinvasive E coli (EIEC);
• Salmonella species microorganisms;
• Thermophillic group of Campylobacter species microorganisms;
• Enteropathogenic E coli (EPEC) and enteroaggregative E coli (EAEC);

Viral agents:

• Group A rotaviruses;
• Genotype 2 noroviruses;
• Group F adenoviruses (Types 40 and 41);
• Astroviruses.
The following causative agents are less widely or not universally spread but are not less important for epidemic outbreaks:
• Vibrio cholerae;
• Yersinia pseudotuberculosis;
• Clostridium diffi cilae;
• Enterotoxigenic E. coli (ETEC), Enterohemorrhagic E. coli (EHEC);
• Genotype 1 Enteroviruses;
• Group C Rotaviruses.

INTENDED USE

Kit A.I.I. Screen Real-TM is a Real-Time test for the qualitative detection and differentiation of Shigella Spp. E.coli, Salmonella spp., Campylobacter spp., Adenovirus F, Rotavirus A, Norovirus 2 genotype, Astrovirus in the biological materials and in the environment. RNA/DNA is extracted from specimens, amplified using RT-amplification and detected using fluorescent reporter dye probes specific for A.I.I. and IC (Internal Control).

PRINCIPLE OF ASSAY

Kit A.I.I. Screen Real-TM is based on three major processes: isolation of RNA/DNA from specimens, reverse transcription of the RNA and Real Time amplification. Test contains an IC which serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition