Corinebacterium diphteriae/tox-genes RT-PCR | B2842-100FRT

Corinebacterium diphteriae/tox-genes RT-PCR | B2842-100FRT from Sacace Biotechnologies is available for delivery

Description:

General information: Real Time PCR kit for detection of Corinebacterium diphteriae

Target Disease Type: Dangerous Infections

Specific Application: Diphtheria

Storage and Shipping : on request

Corinebacterium diphteriae/tox-genes RT-PCR B2842-100FRT DataSheet

INTRODUCTION

Corynebacterium diphtheriae is the pathogenic bacterium that causes diphtheria. C. diphtheriae produces diphtheria toxin which alters protein function in the host by inactivating the elongation factor EF-2. This causes pharyngitis and 'pseudomembrane' in the throat. The diphtheria toxin gene is encoded by a bacteriophage found in toxigenic strains, integrated into the bacterial chromosome.

INTENDED USE

The Corynebacterium diphtheriae / tox-genes Real-TM is a Real-Time PCR test for the qualitative detection and differentiation of Corynebacterium diphtheriae and genes encoding toxins of Corynebacterium diphtheriae and Corynebacterium ulcerans in the biological material (nasopharyngeal swabs, oropharyngeal swabs, swabs from disease sites, germ culture).

PRINCIPLE OF ASSAY

Kit Corynebacterium diphtheriae / tox-genes Real-TM is based on two major processes: DNA is extracted from samples and amplified using real time amplification with fluorescent reporter dye probes specific for rpoB gene site of Corynebacterium diphtheria, gene encoding Corynebacterium diphtheria toxin, gene encoding Corynebacterium ulcerans toxin as well as amplification of Internal Control IC. The test contains an exogenous Internal Control (IC) which serves as an extraction and amplification control for each individually processed specimen and to identify possible reaction inhibition.