Cryptococcus neoformans RT-PCR | F4-100FRT

Cryptococcus neoformans RT-PCR | F4-100FRT from Sacace Biotechnologies is available for delivery

Description:

General information: Real Time PCR Test for detection of Cryptococcus neoformans

Target Disease Type: HIV Associated Kits

Specific Application: HIV Associated Kits

Storage and Shipping : 4 weeks

Cryptococcus neoformans RT-PCR F4-100FRT DataSheet

INTENDED USE

Cryptococcus neoformans Real-TM kit is a Real-Time test for the qualitative detection of Cryptococcus neoformans.

PRINCIPLE OF ASSAY

Cryptococcus neoformans Real-TM kit is a Real-Time test for the qualitative detection Cryptococcus neoformans DNA in the biological material (cerebrospinal fluid, bronchoalveolar lavage, sputum, blood, skin lesions aspirate, viscera biopsy and autopsy material) by using real-time hybridization-fluorescence detection of amplified products. The DNA extraction is carried out with the internal control sample (IC) which helps control the test procedure for each sample.

Cryptococcus neoformans detection by the polymerase chain reaction (PCR) is based on the amplification of the pathogen genome specific region using specific Cryptococcus neoformans primers. In the real-time PCR, the amplified product is detected with the use of fluorescent dyes. These dyes are linked to oligonucleotide probes, which bind specifically to the amplified product during thermocycling.

The real-time monitoring of fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run.  Cryptococcus neoformans DNA amplification is detected on JOE(Yellow)/HEX/Cy3 channel and the IC DNA amplification is detected on FAM (Green) channel.

STABILITY

Cryptococcus neoformans Real-TM Test is stable up to the expiration date indicated on the kit label. The product will maintain performance through the control date printed on the label. Exposure to light, heat or humidity may affect the shelf life of some of the kit components and should be avoided. Repeated thawing and freezing of these reagents should be avoided, as this may reduce the sensitivity.