JCV/BKV Virus RT-PCR Quant (CE) | V71-100FRT

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JCV/BKV Virus RT-PCR Quant | V71-100FRT from Sacace Biotechnologies is available for delivery


General information: Real Time PCR test for quantitative detection of JC Virus and BK virus

Target Disease Type: Neurological Infections

Specific Application: Meningitis

Storage and Shipping : 4 weeks

JCV/BKV Virus RT-PCR Quant (CE) V71-100FRT DataSheet


kit JCV/BKV Virus Real-TM Quant is an in vitro Real Time amplification test for quantitative detection of JC Virus and BK virus in biological material (whole blood, cerebrospinal fluid, urine). The kit is used to study biological material obtained from individuals suspected of progressing multifocal leukoencephalopathy, JC encephalopathy, meningitis, meningoencephalitis, encephalitis caused by the JC virus and BK-associated nephropathy. DNA is extracted from samples, amplified using real time amplification with fluorescent reporter dye probes specific for JCV/BKV and an exogenous Internal Control (IC) The Internal Control serves as an amplification control for each individually processed specimen and to identify any possible reaction inhibition.


kit JCV/BKV Virus Real-TM Quant is based on two major processes: isolation of DNA from specimens and Real Time amplification. Amplification results of JCV/BKV DNA and Internal Control are detected on different channels.

Extraction of DNA from whole blood, cerebrospinal fluid, urine is performed in the presence of an exogenous internal control sample (IC), which allows to monitor all stages of the PCR test for each sample and to evaluate the effect of inhibitors on the final results of the PCR. The quantitative determination of JCV/BKV DNA is possible on the basis of a linear relationship between the initial concentration of the target DNA in the tested sample and the Ct. For the quantitative test, the amplification of DNA from the samples is carried out simultaneously with the DNA calibrators with a known concentration of DNA. Based on the results of the amplification of DNA calibrators, is made a calibration line that allows to calculate the concentration of the target DNA in the clinical samples.

At the stage of amplification, three reactions are simultaneously performed in the same tube. The results of the targets are detected on 3 different fluorescence channels: Internal Control on FAM channel, JCV on Joe channel and BKV on ROX channel.

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