Neisseria gonorrhoeae RT-PCR Quant (CE) | B204-100FRT

Neisseria gonorrhoeae RT-PCR Quant | B204-100FRT from Sacace Biotechnologies is available for delivery

Real Time PCR Kit for quantitative detection of Neisseria gonorrhoeae

Storage & Shipping :

Must be stored at 2-8°C.The kit can be shipped at 2-8°C but should be stored at 2-8°C immediately on receipt. .

Neisseria gonorrhoeae RT-PCR Quant (CE) B204-100FRT DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gon[1]orrhea, mycoplasma, herpes, HIV, HPV, syphilis, gardnerella and trichomoniasis.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

Kit Neisseria gonorrhoeae Real-TM Quant is a test for the quantitative detection of Neisseria gonorrhoeae in the whole blood, tissue, urogenital swabs, urine, prostatic liquid and other biological materials.

PRINCIPLE OF ASSAY

Kit Neisseria gonorrhoeae Real-TM Quant is based on two major processes: isolation of DNA from specimens and Real Time amplification. In real-time PCR, the fluorescent signal is generated from the presence of an olygonucleotide probe specific for target DNA sequence.

The probe contains a fluorescent dye molecule on its 5’ end and a quencher molecule on its 3’ end. The probe hybridizes with one of the chains of the amplified fragment. During synthesis of a complementary chain, Taq DNA polymerase cleaves the probe due to its 5’-3’ nuclease activity. As a result, the fluorescent dye molecule becomes separated from the quencher, and the total fluorescence of reaction volume increases in direct proportion to the number of amplicon copies synthesized during PCR. The fluorescent signal is measured in each cycle of reaction, and the threshold cycle value is determined from the obtained curve. The threshold cycle is proportional to the initial number of DNA copies in a sample, and its value allows quantitative comparisons of analyzed and control samples.

In Neisseria gonorrhoeae Real-TM Quant kit there are 2 independent reactions running in parallel in each tube: the first reaction allows to detect and to quantify the specific fragment of Neisseria gonorrhoeae (Joe/Yellow/Cy3/HEX channel) and the second reaction detects β-actin gene (Fam/Green channel) present in all samples obtained from cells and allows not only to control all analysis steps, but also to estimate sample handling and storage.