Description

anti-Caspase-1 (p20) (mouse), mAb (Casper-1) | AG-20B-0042-C100

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Properties
Clone	Casper-1
Isotype	Mouse IgG1
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant mouse caspase-1.
Application	Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation) Immunohistochemistry: (1:500; paraffin sections) Immunoprecipitation: (1:200)
Crossreactivity	Mouse Rat
Specificity	Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1. Described to cross-react with full-length and activated (p20 fragment) of rat caspase-1 (Lit. #30 & 34).
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.