MagSi-NGSPREP Plus | MDKT000100

Magnetic bead-based MagSi-NGS^PREP Plus offers an efficient solution for both size selection and clean-up of the successive enzymatic reactions in library preparation for NGS applications. The simple and flexible protocol can be adjusted to your specific library preparation kit and NGS instrument. MagSi-NGS^PREP Plus can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent sequencing results.

The MagSi-NGS^PREP Plus kit is optimized for use on Biomek^® Laboratory Automation Workstations and Hamilton^® MicroLab STARline. MagSi-NGS^PREP Plus allows either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80 – 1000 bp with specific reagent volume to sample volume ratio’s. By increasing the volume of MagSi-NGS^PREP Plus, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard undesired fragment sizes.

MagSi-NGS^PREP Plus’ flexible protocols are easy to automate for high-throughput screening.

High quality results

• High recovery of PCR products
• Excellent removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
• Fragment size selection adjustable between 100 and 1000 base pairs
• Guarantees consistent sequencing results

Simplify your routine

• One product for all clean-up and size selection steps in the library preparation work-flow
• Simple bind-wash-elute procedure with short process time
• Protocol easily adjustable for clean-up or size selection using specific reagent-to-sample ratios
• Manual and automate use
• Compatible with standard protocols of common library preparation kits

DNA size distribution curves after left-sided size selection showing a direct comparison between MagSi-NGS^PREP Plus and AMPure^® XP. Four different bead-to-sample ratios were used.

Agarose gel showing 6 independent size selections from a DNA ladder, using ratios of 0.65x (left) and 1.0x (right)