Simian Immunodeficiency Virus (SIV) is a member of the lentivirus genus of the family Retroviridae. Simian Immunodeficiency Virus (SIV) infection of non-human primates is a well-established animal model for studying Human Immunodeficiency Virus (HIV). While SIV infections in their natural host species (African monkeys and chimpanzees) are rarely pathogenic, cross-species SIV infections in macaques are an extensively studied model for HIV pathogenesis, therapeutics and vaccine development.
Specificity: Reacts with antibodies to all major gene products of SIV strains mac, agm, sm and mne. Reacts with human and non-human primate antibodies to HIV-2
Source: SIVmac251 grown in human lymphoid cells
Composition: Derived from electroblotting of sucrose purified, detergent disrupted Simian Immunodeficiency Virus proteins separated on SDS-PAGE gels. Strips contain a serum control band of goat anti-human IgG that reacts when human or non-human primate IgG antibodies are present to control for sample addition.
Contents: One tube of 10 nitrocellulose strips
Store at 2-8 ºC. Keep tightly capped. Allow tube to reach room temperature before opening.
Detection of antibodies to SIV in non-human primate serum or plasma
The nitrocellulose strip was incubated with a 1/100 dilution of a strongly reactive specimen. An alkaline phosphatase-labeled goat anti-human IgG at 0.25 µg/ml was used as a secondary antibody and NBT/BCIP as substrate solution to develop the signal.