Recombinant Protein A/G (r-PAG)

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SKU:
GEN-NRPA14S
Formulation:
lyophilized from 5 mM of PB (4 mM of Na2HPO4, 1 mM of NaH2PO4, pH 7.4)
Mol. Weight:
56.3 kDa
Theoretical pI:
4.74
Theoretical activity:
18.1 U / mg protein (rc-SA: Biotin = 1: 4 (mol: mol))
Resources:
Escherichia coli (E. coli)
Purity:
≥95% by SDS-PAGE analysis
Absorption coefficient:
280nm absorption method, the molar absorption coefficient is 3.74 × 104 (mol / L) -1cm-1 , c (g / L) = A280nm / 0.66
Endotoxin:
≤5EU / mg
Storage condition:
-20°C
Storage period:
3 years
€126.00 - €1,860.00
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Description

The recombinant protein a G protein fusion protein (r-pag) fused 8 Fc binding regions of the antibody, including 5 Fc binding domains from Staphylococcus aureus protein A and 3 Fc binding domains from G protein from Staphylococcus aureus. Streptococcus, and eliminated the cell wall binding domain, albumin binding domain and non-specific binding domain of protein A and protein G. Compared to a single protein A or protein G, it has a range broader binding and can bind to all human IgG subtypes, IgA, IgE, and IgM, but not mouse IgA, IgM, and serum albumin. It is suitable for the extraction and detection of mouse IgG monoclonal antibody. At the same time, the fused r-pag reduced pH dependence and allowed binding to pH5-8.

M : Standard protein marker

Track 1 : r-PAG

For research only!

Recombinant Protein A/G (r-PAG) DataSheet

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