Recombinant Streptavidin (r-SA)

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SKU:
GEN-NRPA05S
Formulation:
lyophilized from 5mM PB (0.5mM Na2HPO4, 4.5mM NaH2PO4, pH ~ 6.0)
Mol. Weight:
16.5 kDa
Resources:
Escherichia coli (E. coli)
Purity:
≥95% by SDS-PAGE analysis
Activity:
≥13 U / mg protein (determined by NUPTEC according to the modified green method)
Storage condition:
-20 °C
Storage period:
3 years
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Description

Streptavidin is a homotetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with high affinity virtually unmatched in nature. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point close to neutrality. Therefore, it has a reducing nonspecific binding level compared to avidin. Streptavidin has been widely applied in various biological fields, such as ELISA, IHC, TRFIA, PCR quantification, single-stranded nucleotide isolation, biomolecule purification, and monoclonal antibody production.

M : protein molecular weight marker

Lane 1: Recombinant streptavidin subunit

Lane 2: recombinant streptavidin homotetramer

For research only!

Recombinant Streptavidin (r-SA) DataSheet

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