Recombinant Streptavidin (r-SA)
- SKU:
- GEN-NRPA05S
- Formulation:
- lyophilized from 5mM PB (0.5mM Na2HPO4, 4.5mM NaH2PO4, pH ~ 6.0)
- Mol. Weight:
- 16.5 kDa
- Resources:
- Escherichia coli (E. coli)
- Purity:
- ≥95% by SDS-PAGE analysis
- Activity:
- ≥13 U / mg protein (determined by NUPTEC according to the modified green method)
- Storage condition:
- -20 °C
- Storage period:
- 3 years
Description
Streptavidin is a homotetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with high affinity virtually unmatched in nature. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point close to neutrality. Therefore, it has a reducing nonspecific binding level compared to avidin. Streptavidin has been widely applied in various biological fields, such as ELISA, IHC, TRFIA, PCR quantification, single-stranded nucleotide isolation, biomolecule purification, and monoclonal antibody production.
M ： protein molecular weight marker
Lane 1: Recombinant streptavidin subunit
Lane 2: recombinant streptavidin homotetramer
For research only！
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