Human Monkeypox virus nucleic acid Test Kit (RT-PCR) 50 tests

The Elanscience Monkeypox virus nucleic acid Test Kit is a real-time polymerase chain reaction (PCR) test intended for the qualitative detection of monkeypox virus in lesion exudate specimens.

Product Features
1. Applied Biosystems™ 7500 Fast Dx Real‑Time PCR Instrument
2. F3L gene of Monkeypox virus
3. LOD:10 copies/μL
Storage
The kit should be stored at -30 to -15℃ protected from light until the expiration date printed on the pouch.

Background

Monkeypox virus  PCR (MPV PCR) is a test procedure for the Monkeypox advised by the CDC to be performed by a real-time PCR assay.

1. The Centers for Disease Control or CDC published this protocol is to describe the procedure used for the detection of Monkeypox virus.
2. DNA in clinical specimens by real-time PCR. This assay detects DNA at varying concentrations, providing a qualitative result of either positive, negative, or inconclusive in the identification of Monkeypox virus infections.

How to perform Monkeypox virus diagnostic testing by real-time PCR?

The methodologies of detecting the monkeypox virus are serology, isolation of pathogen and PCR test. The PCR test is much better in detecting virus for the difficulty in differentiating similar viruses by serology and longer time will be taken by isolation of pathogen.

The real time PCR test represents a significant addition to the battery of tests for the detection of various orthopoxviruses. In light of the recent monkeypox virus transmissions, early detection of the virus is crucial.

Monkeypox NAT controls

Monkeypox virus positive control:

Potential sources for positive controls for this PCR are DNA from Monkeypox virus, or a plasmid containing a cloned PCR target or the NATtrol type controls from Zeptometrix.

Human DNA positive control:

Any source of human DNA that results in a positive reaction with the MPV PVR being used to confirm presence of human DNA is acceptable for use as a positive control.

Human genomic DNA from Gentaur or Zyagen.

Extraction control:

Any human cell line or normal cells can be used with the human DNA primers and probes that results in a positive reaction to confirm the extraction was acceptable.

To prepare positive controls and extraction control, dilute DNA or make aliquots of
human cells (extraction control) with a known cut-off to create a positive control for use
when performing real-time PCR.

a. In a 40 cycle PCR, a positive control should have a CT cut-off value between 22–28
and (if used) a second low positive control should have a cut-off value between 30– 36.

A standard curve can also be used and can be prepared using DNA with a known
concentration. For example, logarithmic dilutions from 0.1 ng/µL to 1 fg/µL are created
from the known positive control and run-in triplicate with the specimens to be tested.

Negative PCR control:

(e.g., no template control (NTC): control specimen containing all
reagents except the target template; molecular grade water is an example of an
appropriate NTC. A NTC is a mechanism to detect cross-contamination of specimens.