Sample Preparation for Mass Spectrometry

  • Proteome Enrichment
  • Not based on immuno-affinity
  • Bead Assisted Sample Prep (BASP™)
  • LC-MS, MALDI Compatible Protocols

High abundance proteins and lipids are well known interferences in mass spectrometry based proteomic analysis. For years the protein depletion toolkit was limited primarily to immuno-affinity chromatography and other biologically-derived tools.

BSG offers two complementary technologies for proteomic researchers to selectively bind or enrich, in order to achieve the best results. By using consumable non-biologically derived beads, researchers will be able to improve efficiency and quantitation essential for expanding mass spectrometry into routine healthcare.

 

AlbuVoid™ LC-MS On-Bead

Albumin depletion plus low abundance protein enrichment, coupled with optimized on-bead digestion (BASP™) protocols for LC-MS serum and plasma proteomics

  • Seamless workflows
  • Unique proteolytic efficiencies
  • Label, label free compatible

References

The Commonality of the Cancer Serum Proteome Phenotype as analyzed by LC-MS/MS and its application to monitor dysregulated wellness.

AlbuVoid™ coupled to on-bead digestion - Tackling the challenges of serum proteomics.

AlbuVoid™ PLUS

Albumin and IgG Depletion From Serum/Plasma for Proteomics

  • IgG removal >90% (70-80% of total Immunoglobulins removed)
  • Albumin removal >95%
  • Seamless and simple < 1 hour protocol
  • Low abundance enrichment equivalent to immuno-affinity
  • Disposable, cost-effective, no column regeneration or cross-contamination
  • Works for most species tested including human, sheep, rat, mouse, bovine
  • On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
  • Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.

HemoVoid™ LC-MS On-Bead

Hemoglobin depletion plus low abundance protein enrichment, coupled with optimized on-bead digestion protocols (BASP™) for LC-MS erythrocyte & blood proteomics

  • Seamless workflows
  • Unique proteolytic efficiencies
  • Label, label free compatible

AlbuSorb™

Selectively Binds Albumin

  • Removes serum albumin >90%
  • Economical small ligand surface architecture (not dye-based), bio-affinity performance
  • Species agnostic

AlbuSorb™ PLUS

Selectively Binds Albumin & Immunoglobulin

  • > 85% Albumin, 85% IgG depleted from 25 μl serum

Reference

For serum exosome enrichment

Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy

HemogloBind™
and
NuGel™ HemogloBind™

Removes Hemoglobin Interference

  • Highly specific for hemoglobin binding
  • depletion from hemolyzed serum, dura, BALF, and whole blood
  • supports biomarker quantitative analysis

References

Panorama of ancient metazoan macromolecular complexes

A novel methodology for the analysis of membrane and cytosolic sub-proteomes of erythrocytes by 2-DE

Cleanascite™

Lipid Adsorption & Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
  • A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
  • Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates

References

Plasma/Serum Protein Biomarkers

For diabetes biomarkers, Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }

Bile Proteomics

Cleanascite™ treatment was indispensable for in-solution digestion methods. { https://pubag.nal.usda.gov/catalog/5599484 }

For MALDI-TOF Analysis

Farina A, Dumonceau JM, Frossard JL. Proteomic Analysis of Human Bile from Malignant Biliary Stenosis Induced by Pancreatic Cancer Journal of Proteome Research.2009; 8(1):159-69

NuGel™ NRicher™ 6

Top-down proteomics enrichment kit

  • 12 differentiated subproteomes, 6 flow-through fractions, and 6 elution fractions
  • Enrich low abundance functional biomarkers for sequence and structural annotation
  • In a rigorous examination of protein complexes, about twice the number of observations were made possible through sub-proteome bias characteristics of NRicher™ 6 { Nature doi:10.1038/nature14877 }
  • Kit includes 6 mixed mode bead chemistries per prep
  • All Top-down proteomic applications
  • Optimize biomarker enrichment after initial enrichment using NRicher™ Mx

NuGel™ NRicher™ Mx

Low Abundance Proteome Enrichment

  • Enriches & normalizes sub-proteomes
  • Compress proteome concentrations
  • Species and tissue agnostic
  • Composite of the NRicher™ 6 mixed mode beads