The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Tribioscience’s ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP levels are measured by the conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive when compared with other methods used for cell viability assays (can detect 100 mammalian cells/well).

Applications:

1. Cell Proliferation
2. Cytotoxicity and Apoptosis
3. HTS

Key Features:

Sensitive and Accurate: As low as 100 cells can be accurately quantified.
Simple and High-Throughput: Simple procedure; Can be readily automated on HTS liquid handling systems.

Storage Conditions:

Store kit at -20℃. Shelf life of three months. Warm up all components (except enzyme) to room temperature before use. Briefly centrifuge all small vials prior to opening.