Hemoglobin Assay Kit
Hemoglobin Assay Kit - Cat Number: 6024 From Chondrex.
Research Field: Colitis, Anemia
Host Origin: N/A
Detection Range: 1 mg/ml-0.031 mg/ml
Sample Type: Serum, Plasma
DESCRIPTION: Assay kit to quantify hemoglobin
FORMAT: 96-well ELISA Plate with removeable strips
ASSAY TYPE: Colorimetric Assay
ASSAY TIME: 15 minutes
STANDARD RANGE: 2 mg/ml to 0.03 mg/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Blood, Serum, and Plasma
RECOMMENDED SAMPLE DILUTIONS: 1:200 (Blood)
CHROMOGEN: N/A (read at 400 nm)
VALIDATION DATA: Intra-Assay (5.7-6.7%)/Inter-Assay (2.7-4.5%)/Spiking Test (103-106%)
Hemoglobin is a heme protein carried by erythrocytes and transports oxygen from the lungs to the rest of the body. Therefore, low hemoglobin
levels in erythrocytes or low erythrocyte numbers in blood deprives the body of oxygen. This condition is defined as anemia. This oxygen
deficit can cause dizziness or headaches, while severe or long-lasting anemia can damage organs in the body and even result in death.
Anemia is also a complication of various inflammatory diseases such as sepsis (1-3) and autoimmune diseases (4, 5), and has been observed
in rodent disease models of inflammatory bowel disease (IBD) (5, 6), colitis (7), sepsis (8), and autoimmune hemolytic anemia (9-12).
As hemoglobin levels correlate to the oxygen carrying-capacity of blood, hemoglobin is a useful marker of anemia to evaluate disease
severity and effectiveness of treatments. A standard hemoglobin assay utilizes the toxic cyanmethemoglobin method. However, Chondrex,
Inc. would like to introduce a safe, non-cyanide hemoglobin assay kit (13, 14). This assay kit correlates well with the cyanmethemoglobin
method and can be used to evaluate anemia in our mouse sepsis model and mouse IBD model, as well as other disease models. Chondrex,
Inc. also provides related products such as FITC-Dextran, mouse anti-bacterial antibody ELISAs, and an HMGB1 Detection ELISA kit. For
more information, please visit www.chondrex.com or contact email@example.com.
NOTES BEFORE USING ASSAY
NOTE 1: It is recommended that the standard and samples be run in duplicate.
NOTE 2: Warm up all buffers to room temperature before use.
NOTE 3: Crystals may form in Wash Buffer, 20X when stored at cold temperatures. If crystals have formed, warm the wash buffer by placing
the bottle in warm water until crystals are completely dissolved.
NOTE 4: Measure exact volume of buffers using a serological pipet, as extra buffer is provided.