Cytochrome C Oxidase Activity Assay | TBS2115

Cytochrome c oxidase [EC 1.9.3.1.] or Complex IV is the fourth complex of the electron Transport Chain located in the mitochondrial, and the principle terminal oxidase of high affinity oxygen in the aerobic metabolism of all animals, plants, yeasts, and some bacteria. This enzyme is probably unique in providing the energy for the cell by coupling of the electron transport through the cytochrome chain with the process of oxidative phosphorylation. The colorimetric assay in this kit is based on observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for detection of mitochondrial outer membrane integrity and for detection of mitochondria in subcellular fractions.

Method:

Colorimetric

Storage Conditions:

The kit ships on wet ice and storage at –20℃ is recommended. When stored unopened, the components in this kit are stable for 24 months. After initial thawing of the 1M Dithiothreitol Solution, divide the solution into undiluted working aliquots (still at 1M concentration) and store at –20℃. sufficient for 100 tests