TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution) | KTC4100

Product Overwiew:

Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution) is a fluorescence imaging tool for staining of Tubulin in mammalian living cells with high specificity and low background.

Background:

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Used for:

N/A

Kit components:

• TubGreen™ (200 uM)
• Buffer T

Features & Benefits:

• Optimized staining protocol for labeling Tubulin in mammalian living cells. The product has been tested in U2OS, Hela, COS-7 and ARPE cell lines. U2OS cell line is preferred. If the sample type is not included in the above cell lines, we can provide experimental services for specific cell lines.
• Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF and PALM).
• Proprietary TubGreen™ (Ex/Em = 500/520 nm)-high specificity, low background and excellent photostability.
• Low levels of cytotoxicity.

Usage Notes:

Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.

Storage buffer:

N/A

Storage instructions:

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.