AffiVET® Bovine Brucella Antibody Rapid Test

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Rapid Test
25 Tests/kit
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Bovine Brucella Antibody rapid test kit

1. Principle

This test card is based on fast immuno-chromatography technique to detect Brucella Ab in bovine serum, plasma and whole blood qualitatively. When testing, Brucella Ab in sample combine with antigen coated by colloid gold forming complexes, moving forward to the other head. When reaching the T-line, the specific antigen on the membrane capture the complexes and appear a T line, C line appears, means the test is valid. The T line appears, means there is Brucella Ab in the sample.

2. Specifications:  25 tests/kit


1)  Brucella Test cassette       25 pieces

2)  Dropper                           25 pieces

3)  Buffer                              1 bottle

4)  Manual                            1 piece

4. Sample preparation

4.1 Whole blood

Collect fresh blood and test immediately; fort anticoagulated whole blood, use for that very day;

4.2 Serum

Collect blood, put at 37℃for 1-2h,until blood coagulation, then centrifuge at 3000r/min for 5-10min, separate the serum. The serum can store for 2-3 days at 4℃, for long term storage, store at -20℃in frozen.

5.Operation procedures

1) Read the manual carefully and return the test card and sample to room temperature before use.

2) Take out test card, use dropper to absorb the sample, drop 1 drop of sample into “S”well on test card, then take bottle with buffer to drop take 2-3 drops into well mark “S”; when the sample quantity is small, can use Micropipette to transfer 5ul sample into well mark “S” first, then add 2-3 drops of buffer to the well.

3) Read the results in 5-10min at room temperature.

6. Results

Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen red color reaction. It means there is no Brucella Ab in sample or the levels is lower than the detection threshold;

Positive(+): Both on T Line and C line being seen red color reaction. It means there is Brucella Ab in sample;

Invalid: No color reaction on C Line. It means the operation is wrong or the test card is invalid.


1) The test card can be used only once at room temperature, do not reuse or use test card out of expiry date.

2) Return all reagents and sample into room temperature before use. Use the test card soon after open the package.

3) Do not use water or other liquid as negative control.

4) Replace at the purchasing place if out of date, bad package or pollution.

5) Be careful when prepare sample, wear glove and mask.

6) The whole blood need add anticoagulants, not frozen; serum sample can store in frozen, avoid repeated freezing and thawing.

7) This product is only for in vitro rapid diagnostic veterinary use, can not be quantified, do only qualitative testing.

8) Please contact the supplier for any questions.

9) The product test results for reference only, for confirmation, please refer to the relevant national standard methods.

Storage and expiry date

Storage:  Store at 2-30℃in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

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1 Review

  • 5

    Easy protocol

    Posted by Lieven Gevaert on 13th Mar 2024

    I love this lateral flow immunochromatographic assay for the qualitative detection of Bovine Brucella antigens in bovine serum/ plasma/whole blood. No false positives or negative reported in the bison and elk populations of the Greater Yellowstone Area.

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