Human Lactate Dehydrogenase A (LDHA) Protein | abx067672

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SKU:: abx067672
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Availability:: Shipped within 5-7 working days.
Clonality:: N/A
Tested Applications:: WB, SDS-PAGE
Host:: E. coli
Conjugation:: Unconjugated
Dry Ice:: No

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Description

Human Lactate Dehydrogenase A (LDHA) Protein. Cat# abx067672. Supplier: abbexa.

Recombinant Lactate Dehydrogenase A (LDHA) is a recombinant Human protein produced in E. coli using Prokaryotic expression.

Target	Lactate Dehydrogenase A (LDHA)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	Reconstitute to the original concentration in ddH₂O. If further dilutions are required, dilute in 20 mM Tris, 150 mM NaCl (pH 8.0) to a concentration of 0.1 to 1 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P00338 (UniProt, ExPASy)
UniProt Entry Name	LDHA_HUMAN
KEGG	hsa:3939
String	9606.ENSP00000445175
Molecular Weight	Calculated MW: 40.8 kDa Observed MW: 33 kDa (determined by SDS-PAGE) Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment	Met1-Phe332 plus VPVC between Ser197 and Val198
Sequence	MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV CVPVWSGMNV AGVSLKTLHP DLGTDKDKEQ WKEVHKQVVE SAYEVIKLKG YTSWAIGLSV ADLAESIMKN LRRVHPVSTM IKGLYGIKDD VFLSVPCILG QNGISDLVKV TLTSEEEARL KKSADTLWGI QKELQF
Tag	N-terminal His-tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity	Not tested
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.