abm | Immortalized Mast Cells (NCL-2) | T0788

abm | Immortalized Mast Cells (NCL-2) | T0788

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Bone Marrow

Organ Type:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Round

Passage Number:

N/A

Population Doubling:

Approximately 30 hours

Seeding Density:

150,000 - 200,000 cells/mL

Markers:

Fc εRI, KIT

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, β-mercaptoethanol to a final concentration of 50 µM, L-glutamine to a final concentration of 2 mM, and Recombinant Mouse IL3 to a final concentration of 5 ng/mL.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Flow cytometry

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C