abm | Immortalized Mast Cells (NCL-2) | T0788
- SKU:
- T0788
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Mast Cells (NCL-2) | T0788
Immortalization Method:
Spontaneous
BioSafety Level:
II
Organism:
Mus musculus
Species:
Mouse
Source Organ:
Bone Marrow
Organ Type:
Bone Marrow
Growth Properties:
Suspension
Morphology:
Round
Passage Number:
N/A
Population Doubling:
Approximately 30 hours
Seeding Density:
150,000 - 200,000 cells/mL
Markers:
Fc εRI, KIT
Donor Age:
N/A
Donor Gender:
Male
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow VIII medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, β-mercaptoethanol to a final concentration of 50 µM, L-glutamine to a final concentration of 2 mM, and Recombinant Mouse IL3 to a final concentration of 5 ng/mL.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) Flow cytometry
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C