abm | Immortalized Mouse Leydig Cells (D-4-4) | T0648

abm | Immortalized Mouse Leydig Cells (D-4-4) | T0648

Immortalization Method:

Derived from transgenic mice expressing polyomavirus large T antigen

BioSafety Level:

II

Organism:

MT-PVLT-10 transgenic mice

Species:

Mouse

Source Organ:

Testicle

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

3β-HSD

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) 50ug/ml Gentamycin used for selection B; 2) RT-PCR used to detect Leydig cell markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C