abm | Immortalized Mouse Proximal Tubule KPT11 Cells | T0640

abm | Immortalized Mouse Proximal Tubule KPT11 Cells | T0640

abm, T0641). It is found that the KPT11 cells have a higher density of gentamicin-binding proteins, such as CLIMP-63. This cell line is a valuable tool for studies relating to the mechanisms of the gentamicin-binding proteins and effects of gentamicin in apoptosis.

Immortalization Method:

Spontaneously immortalization

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Kidney

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

20,000-50,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

RT-PCR and Western blot were used to detect the presence of CLIMP-63

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C