abm | Immortalized Mouse Schwann Cells | T0286

abm | Immortalized Mouse Schwann Cells | T0286

NTR, GFAP and vimentin.

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

(twi/+) Mice

Species:

Mouse

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available from abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Immunofluorescence detection for S100, laminin, and p75 ^NTR, and for GFAP and vimentin markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C