abm | Immortalized Rat Retinal Cells (E1A-NR.3) | T0370

abm | Immortalized Rat Retinal Cells (E1A-NR.3) | T0370

abm (T0575). E1A-NR.3 cells exhibit contact inhibition and anchorage-dependent growth. Researchers may use this cell line to study photoreceptor rescue or replacement paradigm as studies have shown that the Immortalized Rat Retinal Cells (E1A-NR.3) are capable to successfully be transplanted intraocularly.

Immortalization Method:

Serial passaging and transduction with retroviruses carrying 12S portion of replication incompetent adenovirus E1A gene

BioSafety Level:

II

Organism:

PN6 Sprague-Dawley Rat

Species:

Rat

Source Organ:

Retina

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Neuronal

Passage Number:

N/A

Population Doubling:

Doubling time of 24 hours

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Immunoreactivity was assessed in vitro and in vivo for markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C