abm | Immortalized Mouse Retinal Cells (MU-PH1) | T0373

abm | Immortalized Mouse Retinal Cells (MU-PH1) | T0373

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

C57BL/6 Adult Mice

Species:

Mouse

Source Organ:

Retina

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

Vimentin, S-100, glutamine synthetase, nestin, ABCG2,a-tubulin,b-III-tubulin and Ascl1

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

RT-PCR, immunofluorescence and Western blot were used to identify cell markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C