abm | Immortalized Rat Retinal Müller Cells (rMC-1) | T0576

abm | Immortalized Rat Retinal Müller Cells (rMC-1) | T0576

The rMC-1 cell line is derived from stable transformation of SV40 antigen into primary rat retinal Müller cells. The rMC-1 cells resembles its primary counterpart and expresses reactive gliosis marker GFAP and Müller cell-specific marker CRALBP, though at lower levels when compared to the primary cells. This cell line enables mammalian retinal function research, as well as facilitates gene expression studies in Müller cells-neuron and/or Müller cells-endothelial cell interactions.

Immortalization Method:

Serial passaging and Calcium Phosphate mediated transfection with simian virus 40 (SV40) DNA

BioSafety Level:

II

Organism:

Sprague-Dawley Rat

Species:

Rat

Source Organ:

Retina

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Flattened

Passage Number:

N/A

Population Doubling:

12 - 22 hours

Seeding Density:

10,000 - 30,000 cells/cm10²

Markers:

GFAP, CRALBP

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunocytochemistry and immunoblotting were used to detect the presence of glial fibrillary acidic protein (GFAP) and cellular retinaldehyde-binding protein (CRALBP); 2) Immunocytochemistry was used to confirm the nuclear expression of SV40-LTA in the immortalized cells.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C