abm | Immortalized Rat Sympathoadrenal Progenitor Cells (MAH-A3) | T0293

abm | Immortalized Rat Sympathoadrenal Progenitor Cells (MAH-A3) | T0293

abm Z101455), the cells become NGFR-positive post-mitotic sympathetic neurons. MAH-A3 cells are research tools recommended to study the sympathetic development mechanisms.

Immortalization Method:

Serial passaging and transduction with recombinant retroviruses carrying v-myc oncogene

BioSafety Level:

II

Organism:

E14.5 Rat Embryo

Species:

Rat

Source Organ:

Adrenal Glands

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Round

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Grow cells in T25 poly-D-lysine and laminin-coated flasks with the following conditions. The base medium for this cell line is L15-CO2 medium (Lonza; 12-669E). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 50 µM dexamethasone, and Penicillin/Streptomycin Solution to a final concentration of 1%
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Northern blot to confirm v-myc-containing pro-viral RNA transcripts and cell markers; 2) Southern blot to analyze pro-viral transcript integration; 3) Immunofluorescence staining of sympathoadrenal markers.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C