abm | Immortalized Rat Sympathoadrenal Progenitor Cells (MAH-A3) | T0293
Serial passaging and transduction with recombinant retroviruses carrying v-myc oncogene
E14.5 Rat Embryo
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Grow cells in T25 poly-D-lysine and laminin-coated flasks with the following conditions. The base medium for this cell line is L15-CO2 medium (Lonza; 12-669E). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 50 µM dexamethasone, and Penicillin/Streptomycin Solution to a final concentration of 1% Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
1) Northern blot to confirm v-myc-containing pro-viral RNA transcripts and cell markers; 2) Southern blot to analyze pro-viral transcript integration; 3) Immunofluorescence staining of sympathoadrenal markers.
liquid nitrogen or -180C