Biotinylated Lipopolysaccharide from E. coli O111:B4, 0.1 mg, lyophilized

Biotinylated Lipopolysaccharide from E. coli O111:B4, 0.1 mg, lyophilized - Cat Number: 6108 From Chondrex.

Research Field: Bacterial Research

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Biotinylated Lipopolysaccharide (LPS) from E. Coli O111:B4
Lipopolysaccharide (LPS), also known as endotoxin, is the major structural component of the outer membrane of gram-negative bacteria. This glycolipid stimulates the host immune system and plays pathological roles in inflammatory diseases such as bacterial sepsis, inflammatory bowel disorders, lung disease, periodontal disease, and asthma (1-4).

APPLICATION: To facilitate studies on host recognition of LPS, a biotinylated LPS and a streptavidin conjugated probe (enzyme or fluorochrome) can be used for identifying LPS ligands in many applications such as: enzyme immunoassay, western blot, flow cytometry, and fluorescence microscopy (5). In addition, LPS ligand interactions can be evaluated in a pull-down assay as demonstrated with

HMGB1: a late stage mediator of endotoxin shock (6). Chondrex, Inc. also provides purified bovine HMGB1 for use as a positive control for these LPS binding assays (Cat # 9050).

QUANTITY: 0.1 mg
FORM: Lyophilized powder
SOURCE: E. Coli O111:B4
STORAGE: -20°C
STABILITY: 2 years
NOTES: N/A 

REFERENCES:

1. R. Karima, S. Matsumoto, H. Higashi, K. Matsushima, The molecular pathogenesis of endotoxic shock and organ failure. Mol Med Today 5, 123-32 (1999).
2. D. Shi, J. Das, G. Das, Inflammatory bowel disease requires the interplay between innate and adaptive immune signals. Cell Res 16, 70-4 (2006).
3. J. Goldberg, G. Pler, Pseudomonas aeruginosa lipopolysaccharides and pathogenesis. Trends Microbiol 4, 490-4 (1996).
4. B. Bainbridge, S. Coats, R. Darveau, Porphyromonas gingivalis lipopolysaccharide displays functionally diverse interactions with the innate host defense system. Ann Periodontol 7, 29-37 (2002).
5. J. Luk, A. Kumar, R. Tsang, D. Staunton, Biotinylated lipopolysaccharide binds to endotoxin receptor in endothelial and monocytic cells. Anal Biochem 232, 217-24 (1995).
6. H. Hreggvidsdottir, T. Ostberg, H. Wähämaa, H. Schierbeck, A. Aveberger, et al., The alarmin HMGB1 acts in synergy with endogenous and exogenous danger signals to promote inflammation. J Leukoc Biol 86, 655-62 (2009).