OptiPrep™

OptiPrep™ is a sterile, endotoxin-tested, multi-purpose separation medium of 60% iodixanol in water with a density of 1.32 g/ml, ideal for isolating viruses, subcellular organelles, membranes, macromolecules and cells, especially when controlling osmolality is a priority. Iodixanol has undergone rigorous clinical testing and is manufactured under strict EU cGMP compliance.

OptiPrep™ The optimum density gradient medium

OptiPrep™ The ideal density gradient medium for purification and analysis of viruses

Specifications

• Iodixanol         60% (w/v)
• Density            1.320 ± 0.001 g/ml
• Osmolality      ~170 mOsm
• Endotoxin       < 1.0 EU/ml

Applications

• Membrane trafficking and cell signaling analysis
• Endocytosis and exocytosis analysis
• Mammalian and non-mammalian cell purification; routinely achieve maximum yield of non-human lymphocytes with no neutrophil contamination
• Mammalian and nonmammalian organelle purification
• Plasma membrane and domain purification
• Membrane vesicle and cytosol purification
• Virus purification
• Plasma lipoprotein purification
• Protein and protein complex purification
• Plasmid DNA purification
• Ribonucleoprotein purification

Features and properties

• Developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing.
• Non-ionic
• Non-toxic to cells
• Metabolically inert.
• Biologically and physiochemically very similar to Nycodenz® with some key differences:
  • Structurally, an approximate dimer of Nycodenz®
  • Osmolality approximately half that of a Nycodenz® solution of the same density
  • Can be made iso-osmotic at all densities
  • Can be added directly to a cell or organelle suspension without changing its osmolality, facilitating purification in flotation gradients
  • Forms self-generated gradients more quickly than Nycodenz® due to higher molecular mass
• High viability of gradient-purified mammalian and non-mammalian cells
• Effective organelle and membrane vesicle fractionation using either pre-formed or self-generated gradients
• Virus infectivity at least 100x higher than from CsCl gradients
• Better preservation of protein-protein and protein-nucleic acid complexes than with sucrose or glycerol gradients
• Plasma lipoproteins analyzed in self-generated gradients within 4h
• Endotoxin level less guaranteed to be less than 1.0 EU/ml (as determined by limulus amebocyte lysate (LAL) test); however, our goal is to produce batches with endotoxin levels lower or equal to 0.13 EU/ml. Actual density and endotoxin level values for each batch are specified in Certificates of Analysis. 
• Sterility is claimed according to Ph. Eur.
• Manufactured, packaged and released in compliance with:
  • Current EU Guide to Good Manufacturing Practice
  • Fresenius Kabi AS Quality System
  • Fresenius Kabi AS Manufacturing License