OptiPrep™

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SKU:
AXS-1114542
€172.00 - €818.00
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Description

OptiPrep™ is a sterile, endotoxin-tested, multi-purpose separation medium of 60% iodixanol in water with a density of 1.32 g/ml, ideal for isolating viruses, subcellular organelles, membranes, macromolecules and cells, especially when controlling osmolality is a priority. Iodixanol has undergone rigorous clinical testing and is manufactured under strict EU cGMP compliance.

OptiPrep™ The optimum density gradient medium

OptiPrep™ The ideal density gradient medium for purification and analysis of viruses

Specifications

  • Iodixanol         60% (w/v)
  • Density            1.320 ± 0.001 g/ml
  • Osmolality      ~170 mOsm
  • Endotoxin       < 1.0 EU/ml

Applications

  • Membrane trafficking and cell signaling analysis
  • Endocytosis and exocytosis analysis
  • Mammalian and non-mammalian cell purification; routinely achieve maximum yield of non-human lymphocytes with no neutrophil contamination
  • Mammalian and nonmammalian organelle purification
  • Plasma membrane and domain purification
  • Membrane vesicle and cytosol purification
  • Virus purification
  • Plasma lipoprotein purification
  • Protein and protein complex purification
  • Plasmid DNA purification
  • Ribonucleoprotein purification

Features and properties

  • Developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing.
  • Non-ionic
  • Non-toxic to cells
  • Metabolically inert.
  • Biologically and physiochemically very similar to Nycodenz® with some key differences:
    • Structurally, an approximate dimer of Nycodenz®
    • Osmolality approximately half that of a Nycodenz® solution of the same density
    • Can be made iso-osmotic at all densities
    • Can be added directly to a cell or organelle suspension without changing its osmolality, facilitating purification in flotation gradients
    • Forms self-generated gradients more quickly than Nycodenz® due to higher molecular mass
  • High viability of gradient-purified mammalian and non-mammalian cells
  • Effective organelle and membrane vesicle fractionation using either pre-formed or self-generated gradients
  • Virus infectivity at least 100x higher than from CsCl gradients
  • Better preservation of protein-protein and protein-nucleic acid complexes than with sucrose or glycerol gradients
  • Plasma lipoproteins analyzed in self-generated gradients within 4h
  • Endotoxin level less guaranteed to be less than 1.0 EU/ml (as determined by limulus amebocyte lysate (LAL) test); however, our goal is to produce batches with endotoxin levels lower or equal to 0.13 EU/ml. Actual density and endotoxin level values for each batch are specified in Certificates of Analysis. 
  • Sterility is claimed according to Ph. Eur.
  • Manufactured, packaged and released in compliance with:
    • Current EU Guide to Good Manufacturing Practice
    • Fresenius Kabi AS Quality System
    • Fresenius Kabi AS Manufacturing License
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