DirectPCR Lysis Reagent (Mouse Tail) 50ml | 101-T
- SKU:
- 101-T
- Size:
- 50 mL
Description
DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure completely eliminate any solution transfer or tube-opening steps, providing you with substancial extra time.
DirectPCR Lysis Reagent (Mouse Tail) DataSheet
Brief procedure
1. Lyse tails in DirectPCR® Lysis Reagent.
2. Incubate for 45 min at 85°C.
3. PCR genotyping with 1 μl lysates.
Detailed protocols: Tail, Ear, Yolk Sac, and Cultured cells.
DirectPCR® system offers advantages over conventional protocols that include:
· Time saving: Less hands-on time. Crude tail lysates for PCR!
· Money saving: Cost-effective reactions.
· Safe: No organic reagents.
· Environmental: Less waste (organic reagents, tubes, tips, etc...)
· Reliable and efficient: Virtually 100% success rate with high yields.
DirectPCR Lysis Reagent (mouse tail)
101-T 50ml (250 mouse tails).
102-T 100ml (500 mouse tails).
4 Reviews
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effectiveness
I've been using the Direct PCR Lysis Reagent for mouse tail DNA extraction in my research for several months now, and it has significantly enhanced my workflow. This reagent smooth the DNA extraction process by eliminating laborintensive steps like proteinase K digestion and phenol-chloroform extraction. The provided protocol is simple, enabling rapid processing of multiple samples with ease. I've observed that the reagent efficiently lyses mouse tail tissue, generating high-quality DNA ideal for subsequent applications such as PCR and genotyping.
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Good product
DirectPCR lysis reagent is a convenient and efficient reagent for DNA extraction from mouse tail samples. It is suitable for high-throughput genotyping and other molecular biology applications requiring rapid and reliable DNA extraction from mouse tail samples.
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Good product
The DirectPCR Lysis Reagent (Mouse Tail) 50ml, also known as 101-T, is a potent reagent designed for efficient lysis of mouse tail tissue, enabling direct PCR amplification of genomic DNA without the need for DNA purification. This reagent effectively disrupts cell membranes and releases genomic DNA, making it suitable for a wide range of downstream applications in molecular biology and genetics research. I really recommend it!
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Efficiency
I've been consistently impressed by the efficiency of the Direct PCR Lysis Reagent in isolating high-quality genomic DNA from mouse tail samples. The reagent delivers robust results with minimal optimization, allowing for rapid extraction of DNA suitable for downstream applications such as PCR, genotyping, and sequencing.
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