DKW Medium w/ Vitamins

DKW medium is also known as Driver and Kuniyuki Walnut medium. The media was originally developed for walnut micropropagation using nodal explant. Notably, DKW medium contains lower nitrogen and higher sulfate content, potentially influencing plant growth and development.

The Benefits of DKW Media

• Easy to use
• Supply nutrients required by in vitro plants for their proper growth and development
• Lower nitrogen content and higher sulfate content
• Multiple shoot induction
• Callus-mediated shoot regeneration

How To Use DKW Media

Follow the steps below to prepare 1 liter of tissue culture media using DKW media:

1. Take a beaker and add 800 ml of distilled water to it.
2. To the water add 5.36 grams of PCT’s DKW Media, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
3. Make up the volume of the media to 1000ml.
4. Adjust the pH of the media between 5.4-5.8.
5. Add gelling agent (agar 6-8 g/L or gellan gum 3-4 g/L) to the media and keep stirring.=
6. Autoclave the medium for 20 minutes at 15 psi and 121℃.