Description

T4 gp32 Protein is a single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair (1, 2).  It improves the efficiency of reverse transcriptase (RT) during RT-PCR (3), enhances T4 DNA polymerase activity (4), as well as increases the yield of PCR products (5).

Protein Purity

The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining.

Product Source

  1. coli BL21 (DE3) strain expressing T4 gp32 gene.

Product Includes

  • T4 gp32 protein
  • 10X gp32 reaction buffer

1x GP 32 reaction buffer composition

20 mM Tris-acetate
100 mM Potassium acetate
10 mM Magnesium acetate
1 mM DTT
pH 7.8 @ 25ºC

Storage Buffer

50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC

Storage Temperature

–20ºC

Heat Inactivation

65°C for 20 min 

Quality Control assays

T4 gp32 protein is free from detectable nuclease activities.