HHV7 RT-PCR Quant | V17-FRT

HHV7 RT-PCR Quant | V17-FRT from Sacace Biotechnologies is available for delivery

Real Time PCR kit for quantitative detection of Human Herpes Virus 7

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

HHV7 RT-PCR Quant V17-FRT DataSheet

INTENDED USE

kit HHV7 Real-TM Quant is an in vitro Real Time amplification test for quantitative detection of Human Herpes Virus 7 in the biological materials. DNA is extracted from samples, amplified using real time amplification with fluorescent reporter dye probes specific for HHV7, an exogenous Internal Control (IC) and an endogenous Internal Control for the amplification of the human β-globine gene. The Internal Control serves as an amplification control for each individually processed specimen and to identify any possible reaction inhibition.

PRINCIPLE OF ASSAY

kit HHV7 Real-TM Quant is based on two major processes: isolation of DNA from specimens and Real Time amplification. Amplification results of HHV7 DNA are detected on the Joe/HEX/Yellow, β-globine gene DNA used as Internal Control is detected on the Fam/Green channel and the exogenous Internal Control DNA is detected on the ROX/Orange channel.

Extraction of DNA from blood plasma, whole blood and saliva is performed in the presence of an exogenous internal control (IC), which allows to monitor all stages of the PCR test for each sample and to evaluate the effect of inhibitors on the final results of the PCR. Furthermore, when DNA is extracted from whole blood, the DNA portion of the human β-globine gene (endogenous internal control) is amplified. Endogenous internal control (IC glob) allows to control all the steps of the PCR-study as well as the collection and storage of the sample.

The quantitative determination of HHV7 DNA is possible on the basis of a linear relationship between the initial concentration of the target DNA in the tested sample and the Ct. For the quantitative test, the amplification of DNA from the samples is carried out simultaneously with the DNA calibrators with a known concentration of DNA. Based on the results of the amplification of DNA calibrators, is made a calibration line that allows to calculate the concentration of the target DNA in the clinical samples.

At the stage of amplification, three reactions are simultaneously performed in the same tube - amplification of HHV7 DNA, the amplification of the DNA sequence of the exogenous IC and the amplification of the endogenous IC human β-globine gene. The results of the targets are detected on 3 different fluorescence channels.