HSV I/II RT-PCR (CE) | V8-100FRT

HSV I/II RT-PCR (CE) | V8-100FRT from Sacace Biotechnologies is available for delivery

Real Time PCR kit for qualitative detection of Herpes Viruses I and II

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

HSV I/II RT-PCR (CE) V8-100FRT DataSheet

INTRODUCTION

Herpes simplex virus-1 and -2 belong to the Alpha-herpesvirinae of the family Herpesviridae. Their genomes consist of double-stranded DNAs. HSV is one of the most widespread viruses with a prevalence of 95 % and a presence of HSV-2 in 30 – 50 % of human population. HSV-1, the so called oral strain, causes herpes labialis, herpetic stomatitis and keratitis. HSV-2 is called genital strain and causes predominantly genital herpes. In terms of localization of herpes lesions, two forms of herpes are distinguished, the labial herpes with herpes lesions on the upper part of the body (lips, face, mucous membranes of the mouth); and the genital herpes with herpes lesions on the lower part of the body (mucous membranes of the genitals, buttocks, legs). It was believed in the past that only HSV-I could induce labial herpes and only HSV-II could induce genital herpes. The present data disprove this point of view demonstrating tropism common for both types of the virus.

The method of polymerase chain reaction (PCR) in the HSV diagnosis is highly sensitive, specific and less laborious and it has become very important in the laboratory diagnostics of HSV.

INTENDED USE

Kit HSV I/II Real-TM is a test for the qualitative detection of Herpes Simplex Virus I and II in the whole blood, liquor, urogenital swabs, urine, prostatic liquid and other biological materials.

PRINCIPLE OF ASSAY

Kit HSV I/II Real-TM is based on two major processes: isolation of DNA from specimens and Real Time amplification. HSV DNA is extracted from the specimens, amplified using Real-Time amplification and detected fluorescent reporter dye probes specific for HSV DNA and Internal Control. Internal Control (IC) serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition. IC is detected in a channel other than the HSV.