Mouse Antibody Isotyping Card with Heavy & Light Chains | GENISOTYPE04

Mouse Antibody Isotyping card with Heavy & Light Chains. Cat Number: GENISOTYPE04. Supplier: Gentaur.

Description:

This card is used for isotyping rat monoclonal antibodies including IgG1, IgG2a, IgG2b, IgG2c and IgM heavy chains and kappa and lambda light chains. It is simple to use and can get results within 5 minutes.

Instruction of use:

1. Prepare samples to be tested. Samples can be rat monoclonal antibodies in cell culture or in purified forms. The suggested detection range is between 0.5 ug/mL - 10 ug/mL. If antibody concentration is too high, please dilute the sample either using phosphate buffered saline (PBS), pH 7.4 or just simply using deionized water. The total volume of the sample should be more than
180 uL.
2. Open the bag and take the card out. The card should be used within 30 minutes.
3. Write the sample ID and date on the card.
4. The card has 3 oval-shaped wells. Add 60 uL of sample to each well. Wait
2 to 5 minutes until the positive control bands (marked with C) appeared.
5. The band at the corresponding position indicates the antibody isotype, i.e, G1 (IgG1), 2a (IgG2a), 2b (IgG2b), 2c (IgG2c), M (IgM), κ (kappa light chain), λ (lambda light chain).

Typical results using culture supernatants of hybridoma cells

Specifications:

Swissprot: NA

Target Modification: NA

Tested Species : Mouse

Host: Mouse

Tested Applications: rat Antibody Isotyping

Clonality: a set of monoclonal antibodies

Conjugate: Detection antibodies are labelled with colloidal gold

Clone Number: NA

Antibodyisotype: NA

GeneID: NA

Uniprot protein name: NA

Uniprot Gene name: NA

Storage & Shipping :

N/A

Notes:

N/A