Serotonin ELISA | RE59121

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Serotonin ELISA | RE59121 | IBL International

Application: ELISA

Category Type: Catecholamines, Neur

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IHC image of neurons staining for the 5-HT transporter in the raphe nucleus of the rat brainstem . The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary — Add to Cart

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