Selective antibiotic for the neo gene

G418 (Geneticin) : selection antibiotic, endotoxin tested, sterile reagent

G418 also known as G-418, G418 sulfate or Geneticin is an aminoglycoside antibiotic produced by Micromonospora rhodorangea.

G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3' II.

Selection in mammalian cells is usually achieved in 3 to 7 days with concentrations ranging from 400 to 1000 µg/ml.

Cells that are dividing are affected more rapidly than non-dividing cells.

Specifications

Product concentration: 100 mg/ml

CAS number: 108321-42-2

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Purity: ≥ 90% (HPLC)

Endotoxin level: < 0.5 EU/mg

Physicochemical characterization: pH, appearance

Cell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell lines

Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells

Formula: C₂₀H₄₀N₄O₁₀. 2H₂SO₄

Molecular weight: 692.7

Structure:

Contents

G418 is supplied as a sterile filtered solution at 100 mg/ml in HEPES buffer.

This product is available in three pack sizes:

• ant-gn-1: 10 x 1 ml (1 g)
• ant-gn-2: 20 x 1 ml (2 g) 
• ant-gn-5: 1 x 50 ml (5 g)

G418 is shipped at room temperature.

Upon receipt it should be stored at 4°C.

Details

WORKING CONCENTRATIONS

G418 is normally used at a concentration of 400 μg/ml. However, the optimal concentration needs to be determined for your cells.
Suggested concentrations of G418 for selection in some examples of mammalian cells are listed below: