Lipid Removal & Clarification
Cleanascite™
Lipid Adsorption & Clarification
- Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
- Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
- A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
- Suitable alternative to StatSpin LipoClear
- Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
- Clarifies saliva and fecal components
- Exquisite selectivity profile including extracellular vesicle and exosome clearance
- Extends the life of membrane and chromatographic columns.
- Enrichment of delipidated tissue samples
- For downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.
Cleanascite™ is derived through a proprietary formulation of metallic oxide derivatives. Unlike other metallic oxides, Cleanascite™ does not have significant protein binding making its selectivity profile for lipids unique in the bio-research products industry. As a result, it is ideal to clear lipid-associated matrix effects from human sera, bile, ascites, and other high lipid content sample types.
Key References
Plasma/Serum Protein Biomarkers
The authors aimed at simultaneously measuring intact insulin and proinsulin derived C-peptide, to help predict development of diabetes mellitus, as well as in differential diagnosis in cases of hypoglycemia. Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }
Vaccine Development
To evaluate immunogenic response to a vaccine candidate, it is necessary to measure the antibodies from sera; a sample with a diverse lipid profile. In this citation, Cleanascite™ was used in a toxin neutralizing assay to evaluate the influence of cholesterol dependency, on a candidate protein pneumococcal vaccine. { doi: 10.1016/j.vaccine.2012.11.005 }
Bile Proteomics
The authors report methods to overcome the biological variability of analyzing a high number of bile samples. They concluded that delipidation yielded a considerable number of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution digestion methods. { http://dx.doi.org/10.1016/j.jprot.2016.11.021 }
Ascites Monoclonal Antibodies
The researchers determined the role of complement on MAb-mediated protection for four mice Ig subclasses. After centrifugation of ascetic fluid, Cleanascite™ protocol was implemented to remove lipids. { doi: 10.1128/IAI.70.5.2598-2604.2002 }
Cellular Response Applications
The applications and references for the many diverse investigations using Cleanascite™ upstream of cell response measurements are described. { Cleanascite Cell Response Reference Applications }