AffiGEL® Matrix for Organoid culture, Phenol Red-Free, LDEV-Free Equivalent to: CORNING Matrigel Cat No. 356255

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SKU:
AFG-YSG-18/19
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Description

AffiGEL® Matrix for Organoid culture, Phenol Red-Free, LDEV-Free

Equivalent: 356255 | Corning® Matrigel® Matrix for Organoid Culture, Phenol Red-free, LDEV-free

Matrix type: Organoid-specific

Application: Basement membrane matrix for Organoid Culture

 

Product Information 

AffiGEL® Matrix Gel for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture. Verified to support both mouse and human organoid growth and differentiation from healthy and/or diseased tissue, each lot has a specific elastic modulus value indicating the stiffness of the gel formed and is qualified to form stable 3D domes commonly used in organoid culture protocols. Available in 10mL LDEV-free, Phenol Red-free format.

AffiGEL® Matrix Gel for Organoid culture is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

  • Tested for ability to form and maintain stable 3D domes/droplets on multi-well plates.
  • Elastic Modulus (matrix stiffness) measured using a rheometer.
  • Protein concentrations determined by Lowry method
  • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
  • Extensive PCR testing is performed to screen for a number of pathogens
  • Tested and found negative for bacteria, fungi, and mycoplasma
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

AFG-YSG-18

AFG-YSG-19

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1 Review

  • 5

    Effective Organoid Culture Matrix!

    Posted by Isabella Martinez on 4th Jun 2024

    Highly recommend this Matrix! very Excellent! Consistent results in my experiments, with fast delivery and reliable supplier. Highly recommend for researchers studying organoid development and disease modeling! Ensured formation of stable 3D domes for my organoid cultures.

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