Description
Cell Dissociation Reagents (Accutase) | 12679-54 from Nacalai Tesque is available for delivery.
Accutase is a ready to use cell detachment solution, which is non-mammalian, non-bacterial replacement for all applications of trypsin.
Features
- Gentle and efficient dissociation of any adherent cell line
- No mammalian or bacterial components are contained
- No neutralization steps by serum or trypsin inhibitors are required
- Works extremely well on embryonic and neuronal stem cells
Cell Lines Cryopreserved with Accutase™
- hESCs
- vascular endothelial cells
- hepatocyte progenitors
- adherent CHO cells
- 293 cells
- 3T3
- HeLa
- M24 metastatic melanoma
- gliomas D54
- fibroblasts
- hepatocytes
- primary chick embryo neuronal cells
- adherent BHK cells
- L929 cells
- Vero
- NT2
- A375 metastatic melanoma
- HT1080 fibrosarcoma cells
- keratinocytes
- vascular smooth muscle cells
- bone marrow stem cells
- macrophages
- immortalized mouse testicular germ cells
- COS
- MG63
- gliomas U251
- Sf9 insect cells
Applications
Human MG63 Fibrosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase.