Accutase TM

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SKU:: 12679-54
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Condition:: New
Availability:: 5 to 7 Days Shipment
Size:: 100 ml
Storage Conditions:: Freeze
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Description
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Description

Cell Dissociation Reagents (Accutase) | 12679-54 from Nacalai Tesque is available for delivery.

Accutase is a ready to use cell detachment solution, which is non-mammalian, non-bacterial replacement for all applications of trypsin.

Features

Gentle and efficient dissociation of any adherent cell line
No mammalian or bacterial components are contained
No neutralization steps by serum or trypsin inhibitors are required
Works extremely well on embryonic and neuronal stem cells

Cell Lines Cryopreserved with Accutase™

hESCs
vascular endothelial cells
hepatocyte progenitors
adherent CHO cells
293 cells
3T3
HeLa
M24 metastatic melanoma
gliomas D54
fibroblasts
hepatocytes
primary chick embryo neuronal cells
adherent BHK cells
L929 cells

Vero
NT2
A375 metastatic melanoma
HT1080 fibrosarcoma cells
keratinocytes
vascular smooth muscle cells
bone marrow stem cells
macrophages
immortalized mouse testicular germ cells
COS
MG63
gliomas U251
Sf9 insect cells

Applications

Human MG63 Fibrosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase.