Accutase TM

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SKU:
12679-54
Condition:
New
Availability:
5 to 7 Days Shipment
Size:
100 ml
Storage Conditions:
Freeze
Quantity:
Inquire
лв818.20

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Description

Cell Dissociation Reagents (Accutase) | 12679-54 from Nacalai Tesque is available for delivery.

Accutase is a ready to use cell detachment solution, which is non-mammalian, non-bacterial replacement for all applications of trypsin.

Features

  • Gentle and efficient dissociation of any adherent cell line
  • No mammalian or bacterial components are contained
  • No neutralization steps by serum or trypsin inhibitors are required
  • Works extremely well on embryonic and neuronal stem cells

Cell Lines Cryopreserved with Accutase™

  • hESCs
  • vascular endothelial cells
  • hepatocyte progenitors
  • adherent CHO cells
  • 293 cells
  • 3T3
  • HeLa
  • M24 metastatic melanoma
  • gliomas D54
  • fibroblasts
  • hepatocytes
  • primary chick embryo neuronal cells
  • adherent BHK cells
  • L929 cells
  • Vero
  • NT2
  • A375 metastatic melanoma
  • HT1080 fibrosarcoma cells
  • keratinocytes
  • vascular smooth muscle cells
  • bone marrow stem cells
  • macrophages
  • immortalized mouse testicular germ cells
  • COS
  • MG63
  • gliomas U251
  • Sf9 insect cells

Applications

Human MG63 Fibrosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase.

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