Mouse Serum Anti-OVA IgE Antibody Assay Kit

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SKU:
445-3010
Size:
1 kit
Shipping:
Gel Packs
Storage:
-20 C
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Description

Mouse Serum Anti-OVA IgE Antibody Assay Kit - Cat Number: 3010 From Chondrex.

Research Field: Allergy, Immunology

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 25 ng/ml-0.4 ng/ml

Sample Type: Serum, Plasma

Concentration: N/A

Immunogen:

DESCRIPTION: ELISA kit to quantify mouse anti-ovalbumin serum IgE antibodies

FORMAT: 96-well ELISA Plate with removeable strips
TYPE: Sandwich ELISA
ASSAY TIME: 5.5 hours
STANDARD RANGE: 25 - 0.4 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum & Plasma
RECOMMENDED SAMPLE DILUTIONS: 1:10 (at least)
CHROMOGEN: TMB (read at 450 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA: Intra-Assay (7.1%) /Inter-Assay (12.8-5.3%) /Spiking Test (82-92%)
NOTES:

INTRODUCTION

In order to study the pathogenesis of allergic diseases, mice are the most practical experimental animals, due to the variety of inbred strains and transgenic and gene knockout mice that are available. Serum IgE levels are often raised in allergic diseases and parasitic infections, although serum IgE level alone does not reflect the allergic state and the clinical symptoms of the patient. However, it is apparent that a raised serum IgE level aids in the diagnosis of these diseases in humans. As ovalbumin (OVA) is one of the most widely used antigens for studying allergic diseases in mice (4-7), Chondrex, Inc. provides ELISA kits to determine OVA-specific mouse subtype antibodies and to determine mouse total serum antibody levels. For more information, please visit www.chondrex.com.

In general, mouse serum contains various subtypes of antibodies against an antigen, such as IgA, IgM, and IgG, all at higher levels than IgE. Thus, it is difficult to detect IgE antibody levels in serum due to the competition for the antigenic determinant on the antigen by the other subtypes of antibodies. This kit (Catalog # 3010) is designed to detect OVA specific IgE antibodies in mouse serum and can measure antiOVA IgE levels accurately in samples with less than 500 ng/ml of IgE. This kit consists of two components: an anti-IgE monoclonal antibody to capture IgE in serum samples and a biotinylated-OVA for determining OVA-specific IgE antibodies captured by the anti-IgE monoclonal antibody coating the wells. The capture antibody used in this kit reacts equally with both IgEa (Balb/c) and IgEb (C57BL/6) allotypes so it is not necessary to run two separate assays using two independent IgEa and IgEb standards. It does not cross-react with any mouse immunoglobulin subclasses and subtypes (IgA, IgM, IgG, IgG1, IgG2a, IgG2b, IgG2c, and IgG3). 

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