Proteinase K Powder 200mg

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SKU:
506-PKP
  • Proteinase K Powder 200mg
  • Proteinase K Powder 200mg
€235.00
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Description

Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.


Applications: Proteinase K effectively digests native proteins. It can be used to inactivate DNases and RNases when isolating DNA and RNA from tissues or cells.


Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C


Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.


Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

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2 Reviews

  • 5
    good support

    Posted by Bennett on 6th Jan 2022

    the Trust, support and relation of a company with its customers is important for its integrity and Gentaur is good in this support

  • 5
    Original

    Posted by Tripp on 2nd Dec 2021

    Speedy delivery and higher quality

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